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2 edition of Monitoring potato leafroll virus movement in differentially aged potato (Solanum tubersom L.) plants with an immunosorbent direct tissue blotting assay found in the catalog.

Monitoring potato leafroll virus movement in differentially aged potato (Solanum tubersom L.) plants with an immunosorbent direct tissue blotting assay

Jonathan L. Whitworth

Monitoring potato leafroll virus movement in differentially aged potato (Solanum tubersom L.) plants with an immunosorbent direct tissue blotting assay

by Jonathan L. Whitworth

  • 193 Want to read
  • 1 Currently reading

Published .
Written in English

    Subjects:
  • Potato leafroll virus.,
  • Potatoes -- Diseases and pests.,
  • Enzyme-linked immunosorbent assay.,
  • Serology -- Technique.

  • Edition Notes

    Statementby Jonathan L. Whitworth.
    The Physical Object
    Pagination71 leaves, bound :
    Number of Pages71
    ID Numbers
    Open LibraryOL15198595M

      Rubies-Autonell C, Faccioli G, Colombarini A, Detection of potato virus M in potato meristem tips and preliminary results on its eradication. In: Potato Research, 33 Schiessendoppler E, Foschum H, Detection of potato viruses Y, X, A, M, S and potato leafroll virus by dot ELISA. Potato leafroll virus (PLRV) causes a disease of potatoes worldwide and occurs in Western Australia. High levels of infection within a crop reduce returns as the virus .

    Purchase Potato Diseases - 1st Edition. Print Book & E-Book. ISBN , THE STATUS OF POTATO LEAFROLL VIRUS IN KENYA H.K. Were1, R.D. Narla 2, J.H. Nderitu and ann3 1Laboratory of Plant Pathology, Faculty of Agriculture, Graduate School of Kyushu University, , Hakozaki, Higashi-ku, Fukuoka , Japan 2Department of Crop science, University of Nairobi, , Nairobi, Kenya.

      Today, we know that what was described as “curl” was in fact often a complex of several virus diseases including potato leafroll, potato virus Y and also, possibly, potato viruses A and X. In , German scientist Otto Appel coined the term “Blattrollkrankheit” (leafroll disease), thereby distinguishing it from the curl disease complex. Potato virus Y transmission. PVY may be transmitted to potato plants through grafting, plant sap inoculation and through aphid transmission. The most common manner of PVY infection of plant material in the field is through the aphid, and although aphids on their own can directly damage potato plants, it is their role as viral vectors which has the greatest economic impact.


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Monitoring potato leafroll virus movement in differentially aged potato (Solanum tubersom L.) plants with an immunosorbent direct tissue blotting assay by Jonathan L. Whitworth Download PDF EPUB FB2

Monitoring potato leafroll virus movement in differentially aged potato (Solanum tubersom L.) most often within a two week period in early July. In same-age plants inoculated 43 days after planting but 18 days apart, early inoculation produced higher PLRV levels.

Conversely, when same-age plants were inoculated 62 days after planting but 19 Author: Jonathan L. Whitworth. Kathy L. Flanders, David W. Ragsdale and Edward B. Radcliffe, Use of enzyme-linked immunosorbent assay to detect potato leafroll virus in field grown potato, cv.

Russet Burbank, American Potato Journal, /BF, 67, 9, (), ().Cited by: Monitoring potato leafroll virus movement in differentially aged potato (Solanum tubersom L.) plants with an immunosorbent direct tissue blotting assay.

Abstract. Graduation date: Potato leafroll virus (PLRV) causes yield and quality losses in\ud potato. most often within a\ud two week period in early July. In same-age plants. Twelve potato clones with variable degrees of resistance to potato leaf roll virus (PLRV) were co-infected with potato virus X (PVX) or potato virus Y (PVY) before or after inoculations with PLRV.

In some PLRV resistant clones, PVX and PVY facilitated the movement and also increased the concentration of PLRV antigen. The most common virus affecting potatoes in the field worldwide is Potato Leafroll virus (PLRV), belonging to the family Luteoviridae, genius Plerovirus.

There are several molecular methods to detect PLRV including polymerase chain reaction (PCR), Multiplex AmpliDet RNA and double antibody sandwich ELISA (DAS-ELISA).Cited by: In order to investigate the genetic diversity of Potato leafroll virus (PLRV), seven new complete genomic sequences of isolates collected worldwide were compared with the five sequences available in GenBank.

Then, a restricted polymorphic region of the genome was chosen to further analyse new sequences. The sequences of PLRV open reading frames (ORFs) 3 and 4 were also compared with those of.

By P. Hamm and C. Ocamb. Cause The Potato leafroll virus (PLRV) is transmitted by at least 10 species of aphids, in a persistent manner.

Once an aphid acquires the virus, it can transmit it for life, but not pass it on to its offspring. The green peach aphid is the most important vector in our area. Here we present data on the analysis of transgenic Arabidopsisplants expressing Potato leafroll virus17‐kDa movement protein (MP17) fused to green fluorescent protein.

MP17 localizes to secondary branched plasmodesmata (PD) in source but not to simple PD in sink tissues, where MP17 is believed to be degraded by proteolysis. Introduction. Infectious nature of potato leafroll disease described by Quanjer, Van der Lek & Oortwijn Botjes (), virus particles purified by Peters ().

Selected synonyms Potato phloem necrosis virus (Quanjer, ) A virus with RNA-containing isometric. Figure ^—Potato leaves showing symptoms of potato virus X 48 Figure —Potato plant infected with leafroll virus 49 Figure —Phloem necrosis (net necrosis) in tuber following current-season infec- tion with leafroll virus 50 Figure —Leaflet of Green Mountain potato plant infected with mild mosaic virus.

• While virus levels remains low, Potato virus Y (PVYN) relative incidence is increasing o Trend observed since early ’s • PVYN account for 67% of all virus cases in • ~ 80% of virus cases are aphid-transmitted viruses.

Like typical luteoviruses, Potato leafroll virus (PLRV) cannot be transmitted mechanically by rubbing plants with solutions containing virus particles. However, PLRV was found to be mechanically transmissible from extracts of plants that had been inoculated by viruliferous aphids and then post-inoculated with Pea enation mosaic virus-2 (PEMV-2).

Potato leaf roll virus (PLRV) is one of the most important viruses infecting members of Solanaceae family. Among the members of Solanaceae family, potato crop is the most significant host of PLRV.

First report of Potato leafroll virus, Potato virus A, Potato virus X and Potato virus Y in potato in Greenland. de Neergaard 1, L. Munk 2 and S.L.

Nielsen 3 *. Monitoring Potato Leafroll Virus Movement in Differentially Aged Potato (Solanum tuberosum L.) Plants with an Immunosorbent Direct Tissue Blotting Assay.

CHAPTER 1 Literature Review Potato leafroll virus (PLRV) significantly increases the cost of potato production. PLRV causes depressed yields and poor to unacceptable tuber quality in some varieties. Potato leafroll virus (PLRV) was mechanically transmissible when inocula also contained the umbravirus Pea enation mosaic virus-2 (PEMV-2).

In plants infected with PLRV and PEMV-2, PLRV accumulated in clusters of mesophyll cells in both inoculated and systemically infected leaves. An action threshold of green peach aphid,Myzus persicae (Sulzer), apterae per lower leaves is recommended for use in Minnesota to prevent further spread of potato leafroll virus (PLRV) in potato,Solarium tuberosum L.

This threshold was first developed and validated using the PLRV susceptible cultivar Russet Burbank. Here we report experiments to determine if higher aphid densities.

DISTRIBUTION OF POTATO LEAFROLL VIRUS – (PLRV) AND POTATO VIRUS Y – (PVYN) IN A FIELD EXPERIMENT PIGIKIS1, D. HRISTOVA 2 and E. TASHEVA-TERZIEVA 3 1 Agricultural University, BG - Plovdiv, Bulgaria 2 Plant Protection Institute, BG - Kostinbrod, Bulgaria 3 University of Sofia, Faculty of Biology, BG – Sofia, Bulgaria.

Potato virus Y (PVY) is becoming increasingly important in potato growing regions worldwide. The main reason for this is an increase in the incidence of infections with recombinant forms of PVY. In potato, symptoms of primary infection, infection in the growing season, occurs in the youngest leaves.

Leaf margins become necrotic, turning brown and purplish and curl inwards towards the center of the leaf. Secondary infection, which starts from infected potato culls, produces more severe symptoms. Potato virus Y (PVY, family Potyviridae, genus Potyvirus) is considered the most economically damaging potato virus in most countries that produce seed potatoes (Rolot ; Nolte et al.

).The virus is responsible for decreased yield and quality around the world. Most importantly, it is the main reason for rejection of seed lots for certification (Gray et al. ; Nolte et al. ). Potato leafroll virus spread in relation to densities of green peach aphid (Homoptera: Aphididae): implications for management thresholds for Minnesota seed potatoes.

Journal of Economic Entomology, 84(3) Fox L; Biever KD; Toba HH; Duffus JE; Thomas PE, Overwintering and monitoring of potato leafroll virus in some wild crucifers.Introduction. Potato leafroll virus (PLRV) is one of the most prevalent viral diseases of potato in India [].It is the type species of the genus Polerovirus, in the family Luteoviridae [], a group of phloem limited plant virus is tuber borne, not sap transmitted, transmitted efficiently by aphid in a circulative non-propagative manner [6, 16].